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Addgene

pCLX-UBI-GFPf
(Plasmid #27248)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27248 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCLX
  • Backbone size w/o insert (bp) 7879
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Top10 or HB101
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFPf
  • Species
    Aequorea
  • Insert Size (bp)
    792

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR4 (destroyed during cloning)
  • 3′ cloning site attR2 (destroyed during cloning)
  • 5′ sequencing primer ctagtaaagcttctgcaggt
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Clontech

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLX-UBI-GFPf was a gift from Patrick Salmon (Addgene plasmid # 27248 ; http://n2t.net/addgene:27248 ; RRID:Addgene_27248)