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Addgene

pCLX-UBI-Tred
(Plasmid #27246)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27246 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCLX
  • Backbone size w/o insert (bp) 7932
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Top10 or HB101
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TurboRFP
  • Species
    Entacmaea
  • Insert Size (bp)
    693

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR4 (destroyed during cloning)
  • 3′ cloning site attR2 (destroyed during cloning)
  • 5′ sequencing primer ctagtaaagcttctgcaggt
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Evrogen
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLX-UBI-Tred was a gift from Patrick Salmon (Addgene plasmid # 27246 ; http://n2t.net/addgene:27246 ; RRID:Addgene_27246)

  • For your References section:

    Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior. Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ. Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w. 10.1038/s41467-017-01046-w PubMed 29079819
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