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Addgene

pCLX-UBI-GFP
(Plasmid #27245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCLX
  • Backbone size w/o insert (bp) 7860
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Top10 or HB101
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    Aequorea
  • Insert Size (bp)
    717

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR4 (destroyed during cloning)
  • 3′ cloning site attR2 (destroyed during cloning)
  • 5′ sequencing primer ttctgcaggtcgactctaga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLX-UBI-GFP was a gift from Patrick Salmon (Addgene plasmid # 27245 ; http://n2t.net/addgene:27245 ; RRID:Addgene_27245)

  • For your References section:

    Generation of human cell lines using lentiviral-mediated genetic engineering. Salmon P. Methods Mol Biol. 2013;945:417-48. doi: 10.1007/978-1-62703-125-7_25. 10.1007/978-1-62703-125-7_25 PubMed 23097121
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