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Addgene

pEF-BOS huTBK1 K38M Flag-His
(Plasmid #27240)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27240 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEF-BOS
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TANK-binding kinase 1
  • Alt name
    TBK1
  • Alt name
    NAK
  • Alt name
    T2K
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2190
  • Mutation
    Kinase dead: Catalytic lysine changed to alanine (K38M)
  • GenBank ID
    NP_037386
  • Entrez Gene
    TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)
  • Tags / Fusion Proteins
    • Flag (C terminal on backbone)
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer EF1a-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-BOS huTBK1 K38M Flag-His was a gift from Kate Fitzgerald (Addgene plasmid # 27240)
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