pFCK(1.3)tdimer2W
(Plasmid #27233)

• Depositing Lab: Pavel Osten
• Publication: Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11.

Backbone

• Vector backbone: pFUGW
• Backbone size w/o insert (bp): 9066
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DsRed2
• Tag / Fusion Protein:
  • tandem dimer RFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expresses tandem dimer RFP under the mouse α-CaMKII promoter (1.3kb fragment). Similar to plasmid # 27230, except for fluorescent tag.

Note that differences between Addgene's sequencing result and the sequence from the depositing scientist are due to use of theoretical maps. Please utilize Addgene's sequence when available.

EcoRI and BstBI sites are not present according to Addgene's sequencing results.