AAV-CK(1.3)GW
(Plasmid
#27227)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV-MCS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4600
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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Tag
/ Fusion Protein
- EGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses EGFP under the mouse α-CaMKII promoter (1.3kb fragment)
Note that differences between Addgene's sequencing result and the sequence from the depositing scientist are due to use of theoretical maps. Please utilize Addgene's sequence when available.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-CK(1.3)GW was a gift from Pavel Osten (Addgene plasmid # 27227 ; http://n2t.net/addgene:27227 ; RRID:Addgene_27227) -
For your References section:
Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie TW, Helmchen F, Denk W, Brecht M, Osten P. Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11. 10.1073/pnas.0407976101 PubMed 15608064
Map uploaded by Addgene staff.
