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Addgene

pEF-BOS MDA5
(Plasmid #27225)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27225 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEF-BOS
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    melanoma differentiation associated protein-5
  • Alt name
    MDA5
  • Alt name
    Helicard
  • Alt name
    IFIH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • GenBank ID
    NP_071451
  • Entrez Gene
    IFIH1 (a.k.a. AGS7, Hlcd, IDDM19, IMD95, MDA-5, MDA5, RLR-2, SGMRT1)
  • Tags / Fusion Proteins
    • Flag (C terminal on backbone)
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (destroyed during cloning)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer G-CSF-R (5'-GATGGGGAACACTGCTGTTTA-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-BOS MDA5 was a gift from Kate Fitzgerald (Addgene plasmid # 27225)

  • For your References section:

    The RNA helicase Lgp2 inhibits TLR-independent sensing of viral replication by retinoic acid-inducible gene-I. Rothenfusser S, Goutagny N, DiPerna G, Gong M, Monks BG, Schoenemeyer A, Yamamoto M, Akira S, Fitzgerald KA. J Immunol. 2005 Oct 15. 175(8):5260-8. PubMed 16210631
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