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Addgene

pCMV5B ActRIB-HA T206D
(Plasmid #27223)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5B
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ActRIB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    T206D
  • GenBank ID
    U14722
  • Entrez Gene
    ACVR1B (a.k.a. ACTRIB, ACVRLK4, ALK4, SKR2)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5B ActRIB-HA T206D was a gift from Joan Massague (Addgene plasmid # 27223 ; http://n2t.net/addgene:27223 ; RRID:Addgene_27223)

  • For your References section:

    Activation of signalling by the activin receptor complex. Attisano L, Wrana JL, Montalvo E, Massague J. Mol Cell Biol. 1996 Mar . 16(3):1066-73. PubMed 8622651
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