GFP-TREX1(D18N)
(Plasmid #27220)

• Depositing Lab: Judy Lieberman
• Publication: Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TREX1
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: D18N sequence 52-GAC changed to AAC, resulting in Aspartic acid to Asparagine change.
• Entrez Gene: TREX1 (a.k.a. AGS1, CRV, DRN3, HERNS, RVCLS)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry