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Addgene

GFP-TREX1
(Plasmid #27219)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27219 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TREX1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    n/a
  • Entrez Gene
    TREX1 (a.k.a. AGS1, CRV, DRN3, HERNS, RVCLS)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-TREX1 was a gift from Judy Lieberman (Addgene plasmid # 27219 ; http://n2t.net/addgene:27219 ; RRID:Addgene_27219)

  • For your References section:

    The cytosolic exonuclease TREX1 inhibits the innate immune response to human immunodeficiency virus type 1. Yan N, Regalado-Magdos AD, Stiggelbout B, Lee-Kirsch MA, Lieberman J. Nat Immunol. 2010 Nov . 11(11):1005-13. 10.1038/ni.1941 PubMed 20871604
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