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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFLAG-CMV4
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTREX1
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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Mutationn/a
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Entrez GeneTREX1 (a.k.a. AGS1, CRV, DRN3, HERNS, RVCLS)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer C-CMV-30
- 3′ sequencing primer C-CMV-24 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-TREX1 was a gift from Judy Lieberman (Addgene plasmid # 27218 ; http://n2t.net/addgene:27218 ; RRID:Addgene_27218) -
For your References section:
The cytosolic exonuclease TREX1 inhibits the innate immune response to human immunodeficiency virus type 1. Yan N, Regalado-Magdos AD, Stiggelbout B, Lee-Kirsch MA, Lieberman J. Nat Immunol. 2010 Nov . 11(11):1005-13. 10.1038/ni.1941 PubMed 20871604