pBSDT-AII
(Plasmid #27179)

• Depositing Lab: Yoh Wada
• Publication: Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Backbone

• Vector backbone: pBluescript II
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2800
• Vector type: Mammalian Expression, Mouse Targeting
• Selectable markers: negative selection by diphtheria toxin A

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MC1 promoter diphtheria toxin-A fragment
• Alt name: DT-A
• Species: synthetic
• Insert Size (bp): 1100

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 forward, T7
• 3′ sequencing primer: M13 reverse, T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DT-A fragment was obtained from Gibco (discontinued). The original must came from Dr. Shin-ichi Aizawa of RIKEN.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MCS in the pBSDT-AII is extensibly modified by synthetic oligos to create multiple rare restriction sites