pCGN-ELK-1 S383A
(Plasmid #27160)

• Purpose: For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain
• Depositing Lab: Ron Prywes
• Publication: Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-49.

Backbone

• Vector backbone: pCGN
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ELK1
• Species: H. sapiens (human)
• Mutation: TAD mutant: S383A
• Entrez Gene: ELK1
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry