Tet-O-FUW-Ascl1
(Plasmid #27150)

• Depositing Lab: Marius Wernig
• Publication: Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Backbone

• Vector backbone: TetO-FUW
• Backbone size w/o insert (bp): 8393
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: The depositing laboratory used JM109 (Promega). The plasmid is provided in NEB Stable to reduce the chance of recombination.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ascl1
• Alt name: Mash1
• Species: M. musculus (mouse)
• Insert Size (bp): 696
• Entrez Gene: Ascl1 (a.k.a. ASH1, Mash1, bHLHa46)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry