pGMCS-0X-P38-PanBmGFP
(Plasmid #27127)

• Depositing Lab: Sabine Ehrt
• Publication: Gandotra et al PLoS Pathog. 2010 Aug 12;6(8):e1001040. doi: 10.1371/journal.ppat.1001040

Backbone

• Vector backbone: pGMCS
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PanB
• Alt name: Probable 3-methyl-2-oxobutanoate hydroxymethyltransferase
• Species: Mycobacterium tuberculosis
• Insert Size (bp): 842
• Tags / Fusion Proteins:
  • Linker (SGSGSG) (C terminal on insert)
  • mGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: Gateway Cloning (att) (unknown if destroyed)
• 3′ cloning site: Gateway Cloning (att) (unknown if destroyed)
• 5′ sequencing primer: M13_puc_Rev
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • PanBmGFP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry