pG14-MS2-GFP
(Plasmid #27117)

• Depositing Lab: Robert Singer
• Publication: Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Backbone

• Vector backbone: pG14
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MS2-binding protein
• Species: bacteriophage MS2
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • HA (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GPDpro-F
• 3′ sequencing primer: GFP-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An episomal yeast expression plasmid containing the MS2-binding protein fused to GFP. This protein also contains a NLS and a HA tag. The auxotropic marker is LEU2.

There is a 14aa gap in the MS2 sequence starting at aa#67 when compared to GenBank ID: NP_040648.1