pEF5B-FRT-GFP-αTAT1[D157N]
(Plasmid #27100)

• Depositing Lab: Maxence Nachury
• Publication: Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. ():.

Backbone

• Vector backbone: pEF5B-FRT-LAP-DEST
• Backbone size w/o insert (bp): 5971
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: αTubulin K40 acetyltransferase
• Alt name: αTAT1
• Species: H. sapiens (human)
• Insert Size (bp): 999
• Mutation: D157N
• GenBank ID: NM_024909
• Entrez Gene: ATAT1 (a.k.a. C6orf134, MEC17, Nbla00487, TAT, alpha-TAT, alpha-TAT1)
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • S-tag (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: LR reaction (not destroyed)
• 3′ cloning site: LR reaction (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry