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Addgene

pBiFC-VN155(I152L)
(Plasmid #27097)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27097 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMyc-CMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3791
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VN155(I152L)
  • Alt name
    Venus (1-154, I152L)
  • Species
    Aequorea victoria
  • Insert Size (bp)
    462
  • Mutation
    I152L
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To be published by Biotechniques in December. Please suggest the citation of our Biotechniques article. Also, this will be used as a replacement of pBiFC-VN173.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiFC-VN155(I152L) was a gift from Chang-Deng Hu (Addgene plasmid # 27097 ; http://n2t.net/addgene:27097 ; RRID:Addgene_27097)
  • For your References section:

    An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Nov . 49(5):793-805. 10.2144/000113519 PubMed 21091444