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pGV13 (CK2alpha K68M, CK2beta)
(Plasmid #27094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBI
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4358
  • Vector type
    Mammalian Expression ; tetracycline-regulated expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CK2beta
  • Alt name
    CK2β
  • Alt name
    Casein Kinase II beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Entrez Gene
    CSNK2B (a.k.a. CK2B, CK2N, CSK2B, Ckb1, Ckb2, G5A, POBINDS)
  • Promoter bidirectional tet-responsive promoter
  • Tag / Fusion Protein
    • Myc on CK2beta (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer Bglob-intron-R
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CK2alpha K68M
  • Alt name
    CK2α
  • Alt name
    Casein Kinase II alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1429
  • Mutation
    K68M on CK2alpha
  • Entrez Gene
    CSNK2A1 (a.k.a. CK2A1, CKII, Cka1, Cka2, OCNDS)
  • Promoter bidirectional tet-responsive promoter
  • Tag / Fusion Protein
    • HA on CK2alpha (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGV13 (CK2alpha K68M, CK2beta) was a gift from David Litchfield (Addgene plasmid # 27094 ; http://n2t.net/addgene:27094 ; RRID:Addgene_27094)

  • For your References section:

    Protein kinase CK2 is a constitutively active enzyme that promotes cell survival: strategies to identify CK2 substrates and manipulate its activity in mammalian cells. Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, Litchfield DW.. Methods Enzymol. 2010;484:471-93. 10.1016/B978-0-12-381298-8.00023-X PubMed 21036246
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