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pGV15 (CK2alpha K68M)
(Plasmid #27089)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRC/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5542
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CK2alpha K68M (kinase dead)
  • Alt name
    CK2α
  • Alt name
    Casein Kinase II alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1477
  • Mutation
    K68M (kinase dead)
  • Entrez Gene
    CSNK2A1 (a.k.a. CK2A1, CKII, Cka1, Cka2, OCNDS)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGV15 (CK2alpha K68M) was a gift from David Litchfield (Addgene plasmid # 27089 ; http://n2t.net/addgene:27089 ; RRID:Addgene_27089)

  • For your References section:

    Protein kinase CK2 is a constitutively active enzyme that promotes cell survival: strategies to identify CK2 substrates and manipulate its activity in mammalian cells. Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, Litchfield DW.. Methods Enzymol. 2010;484:471-93. 10.1016/B978-0-12-381298-8.00023-X PubMed 21036246
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