pAB46 (CK2beta)
(Plasmid #27085)

• Depositing Lab: David Litchfield
• Publication: Turowec et al Methods Enzymol. 2010;484:471-93.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CK2beta
• Alt name: CK2β
• Alt name: Casein Kinase II beta
• Species: H. sapiens (human)
• Insert Size (bp): 700
• Entrez Gene: CSNK2B (a.k.a. CK2B, CK2N, CSK2B, Ckb1, Ckb2, G5A, POBINDS)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry