pCXLE-EGFP
(Plasmid #27082)

• Purpose: Integration-free (episomal) expression of EGFP
• Depositing Lab: Shinya Yamanaka
• Publication: Okita et al Nat Methods. 2011 May;8(5):409-12

Backbone

• Vector backbone: pCXLE
• Backbone size w/o insert (bp): 10180
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 732

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCEP4 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
• Articles Citing this Plasmid:
  • 54 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry