pCXLE-hMLN
(Plasmid #27079)

• Purpose: Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG
• Depositing Lab: Shinya Yamanaka
• Publication: Okita et al Nat Methods. 2011 May;8(5):409-12

Backbone

• Vector backbone: pCXLE
• Backbone size w/o insert (bp): 10180
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C-MYC, LIN28, NANOG
• Species: H. sapiens (human)
• Insert Size (bp): 3104
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCEP4 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. Mutation(s) made by Provider lab: Three genes are connected with the foot-and-mousht desease virus 2A self-cleaving sequence.
• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry