pGMCZ-PrpoB
(Plasmid
#27073)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDE43-MCZ
- Backbone size w/o insert (bp) 5390
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionszeocin (25 ug/ml)
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameP-rpoB
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SpeciesM. smegmatis
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Insert Size (bp)4548
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMCZ-PrpoB was a gift from Dirk Schnappinger (Addgene plasmid # 27073 ; http://n2t.net/addgene:27073 ; RRID:Addgene_27073) -
For your References section:
Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase. Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D. Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149 10.1093/nar/gkq1149 PubMed 21075796
Map uploaded by the depositor.
