pGMEH-P38-luxAB-ssrAec
(Plasmid
#27062)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27062 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDE43-MEH
- Backbone size w/o insert (bp) 7843
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP#38-luxAB-ssrAec
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Speciessynthetic gene
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Insert Size (bp)2652
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMEH-P38-luxAB-ssrAec was a gift from Dirk Schnappinger (Addgene plasmid # 27062 ; http://n2t.net/addgene:27062 ; RRID:Addgene_27062) -
For your References section:
Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase. Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D. Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149 10.1093/nar/gkq1149 PubMed 21075796