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Addgene

pMSCV FHA-Stop
(Plasmid #27050)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27050 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy-related 12 (yeast)
  • Alt name
    Apg12l
  • Alt name
    Atg12l
  • Alt name
    Atg12
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    530
  • Mutation
    changed glycine 141 to stop codon.
  • GenBank ID
    NM_026217
  • Entrez Gene
    Atg12 (a.k.a. 4931423H11Rik, A330058M13Rik, Apg12l, Atg12l)
  • Tags / Fusion Proteins
    • flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV FHA-Stop was a gift from Jayanta Debnath (Addgene plasmid # 27050 ; http://n2t.net/addgene:27050 ; RRID:Addgene_27050)

  • For your References section:

    ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death. Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath J. Cell. 2010 Aug 20. 142(4):590-600. 10.1016/j.cell.2010.07.018 PubMed 20723759
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