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pQCXIH-Flag Smad3 AAGY
(Plasmid #27027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIH
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    P181A P182A
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site blunt end cloning (destroyed during cloning)
  • 3′ cloning site blunt end cloning (destroyed during cloning)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA PCRed from pCS2-Flag Smad3 with 5' primer containing Flag tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIH-Flag Smad3 AAGY was a gift from Joan Massague (Addgene plasmid # 27027 ; http://n2t.net/addgene:27027 ; RRID:Addgene_27027)

  • For your References section:

    Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias MJ, Erdjument-Bromage H, Tempst P, Massague J. Mol Cell. 2009 Nov 13. 36(3):457-68. 10.1016/j.molcel.2009.09.043 PubMed 19917253
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