MSCV-PM mir shLEF1 b
(Plasmid
#27018)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV
- Backbone size w/o insert (bp) 6300
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemir LEF1 1b
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Insert Size (bp)100
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
shRNA sequence is 5'-GAGTTATTCCGGGTACATA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-PM mir shLEF1 b was a gift from Joan Massague (Addgene plasmid # 27018 ; http://n2t.net/addgene:27018 ; RRID:Addgene_27018) -
For your References section:
WNT/TCF signaling through LEF1 and HOXB9 mediates lung adenocarcinoma metastasis. Nguyen DX, Chiang AC, Zhang XH, Kim JY, Kris MG, Ladanyi M, Gerald WL, Massague J. Cell. 2009 Jul 10. 138(1):51-62. 10.1016/j.cell.2009.04.030 PubMed 19576624