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Addgene

pCI HA NEDD4 DD
(Plasmid #26999)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26999 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEDD4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Mutation
    C867A
  • Entrez Gene
    NEDD4 (a.k.a. NEDD4-1, RPF1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI HA NEDD4 DD was a gift from Joan Massague (Addgene plasmid # 26999 ; http://n2t.net/addgene:26999 ; RRID:Addgene_26999)

  • For your References section:

    Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias MJ, Erdjument-Bromage H, Tempst P, Massague J. Mol Cell. 2009 Nov 13. 36(3):457-68. 10.1016/j.molcel.2009.09.043 PubMed 19917253
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