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Psicheck2 Sox4 mut 3'UTR oligo
(Plasmid #26988)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox4 mut UTR oligo
  • Alt name
    Sox4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Mutation
    bp 449-509 of SOX4 3'UTR; mut @ bp 483
  • Entrez Gene
    SOX4 (a.k.a. CSS10, EVI16)
  • Tag / Fusion Protein
    • Renilla Luciferase (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Rluc-F
  • 3′ sequencing primer psiCHECK2-R (CGAGGTCCGAAGACTCATTT)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TTGCTTCTT => GGGCTGAGG, the mutation is in miR33 target sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Psicheck2 Sox4 mut 3'UTR oligo was a gift from Joan Massague (Addgene plasmid # 26988 ; http://n2t.net/addgene:26988 ; RRID:Addgene_26988)

  • For your References section:

    Endogenous human microRNAs that suppress breast cancer metastasis. Tavazoie SF, Alarcon C, Oskarsson T, Padua D, Wang Q, Bos PD, Gerald WL, Massague J. Nature. 2008 Jan 10. 451(7175):147-52. 10.1038/nature06487 PubMed 18185580
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