pRetroSuper-shYes
(Plasmid
#26987)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRetroSuper
- Backbone size w/o insert (bp) 6401
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshRNA Yes
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Alt nameYes
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SpeciesH. sapiens (human)
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Insert Size (bp)64
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Entrez GeneYES1 (a.k.a. HsT441, P61-YES, Yes, c-yes)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
shRNA sequence - 5'-GTGACAATGTGAAACACTA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRetroSuper-shYes was a gift from Joan Massague (Addgene plasmid # 26987 ; http://n2t.net/addgene:26987 ; RRID:Addgene_26987) -
For your References section:
Latent bone metastasis in breast cancer tied to Src-dependent survival signals. Zhang XH, Wang Q, Gerald W, Hudis CA, Norton L, Smid M, Foekens JA, Massague J. Cancer Cell. 2009 Jul 7. 16(1):67-78. 10.1016/j.ccr.2009.05.017 PubMed 19573813