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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE-hygro
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Backbone manufacturerAddgene Plasmid 1765
- Backbone size w/o insert (bp) 5558
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-Src
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Alt namepp60-c-src
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SpeciesG. gallus (chicken)
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Insert Size (bp)1600
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GenBank IDV00402
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Entrez GeneCSK (a.k.a. c-Src)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE-5
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank: V00402.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-Src-Rescue was a gift from Joan Massague (Addgene plasmid # 26983 ; http://n2t.net/addgene:26983 ; RRID:Addgene_26983) -
For your References section:
Latent bone metastasis in breast cancer tied to Src-dependent survival signals. Zhang XH, Wang Q, Gerald W, Hudis CA, Norton L, Smid M, Foekens JA, Massague J. Cancer Cell. 2009 Jul 7. 16(1):67-78. 10.1016/j.ccr.2009.05.017 PubMed 19573813