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Addgene

pBABE-Src-Dasatinib-resistant
(Plasmid #26980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26980 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone manufacturer
    Addgene Plasmid 1765
  • Backbone size w/o insert (bp) 5558
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Src
  • Alt name
    pp60-c-src
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1600
  • Mutation
    T338I
  • GenBank ID
    V00402
  • Entrez Gene
    SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE-5
  • 3′ sequencing primer pBABE-3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dasatinib resistant mutant.

GenBank: V00402.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-Src-Dasatinib-resistant was a gift from Joan Massague (Addgene plasmid # 26980 ; http://n2t.net/addgene:26980 ; RRID:Addgene_26980)

  • For your References section:

    Latent bone metastasis in breast cancer tied to Src-dependent survival signals. Zhang XH, Wang Q, Gerald W, Hudis CA, Norton L, Smid M, Foekens JA, Massague J. Cancer Cell. 2009 Jul 7. 16(1):67-78. 10.1016/j.ccr.2009.05.017 PubMed 19573813
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