deltaNp63alpha-FLAG
(Plasmid #26979)

• Depositing Lab: David Sidransky
• Publication: Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

Backbone

• Vector backbone: pcDNA3.1/hygro
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5597
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ΔNP63alpha
• Alt name: p63
• Species: H. sapiens (human)
• Mutation: dominant negative isoform of p63; aa 1–587
• Entrez Gene: TP63 (a.k.a. AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's quality control sequence shows an S59G mutation in the insert. The depositing lab does not believe this mutation affects function.