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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 Hygro
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5597
- Total vector size (bp) 7025
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAp63gamma
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SpeciesH. sapiens (human)
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Entrez GeneTP63 (a.k.a. AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)
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Tag
/ Fusion Protein
- 3XHA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TAp63gamma-(3X)HA was a gift from David Sidransky (Addgene plasmid # 26977 ; http://n2t.net/addgene:26977 ; RRID:Addgene_26977) -
For your References section:
TAp63gamma regulates hOGG1 and repair of oxidative damage in cancer cell lines. Upadhyay S, Chatterjee A, Trink B, Sommer M, Ratovitski E, Sidransky D. Biochem Biophys Res Commun. 2007 May 18. 356(4):823-8. 10.1016/j.bbrc.2007.01.168 PubMed 17399686