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Sgf73_Sgf11/PCDFDuet
(Plasmid #26964)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3781
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgf11
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    300
  • GenBank ID
    NM_001183861
  • Entrez Gene
    SGF11
  • Entrez Gene
    SGF73 (a.k.a. YGL066W, SCA7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 promotor 1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sgf 73(1-96) cloned in the second frame by NdeI+BglII for coexpression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sgf73_Sgf11/PCDFDuet was a gift from Cynthia Wolberger (Addgene plasmid # 26964 ; http://n2t.net/addgene:26964 ; RRID:Addgene_26964)

  • For your References section:

    Structural insights into the assembly and function of the SAGA deubiquitinating module. Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, Wolberger C. Science. 2010 May 21. 328(5981):1025-9. 10.1126/science.1190049 PubMed 20395473
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