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Addgene

Sus1/PET32a
(Plasmid #26963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PET32a
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Sus1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    291
  • Mutation
    with TEV site to cleave the N-terminal his+Trx tag
  • GenBank ID
    NM_001184520
  • Entrez Gene
    SUS1 (a.k.a. YBR111W-A)
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • Trx (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sus1/PET32a was a gift from Cynthia Wolberger (Addgene plasmid # 26963 ; http://n2t.net/addgene:26963 ; RRID:Addgene_26963)

  • For your References section:

    Structural insights into the assembly and function of the SAGA deubiquitinating module. Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, Wolberger C. Science. 2010 May 21. 328(5981):1025-9. 10.1126/science.1190049 PubMed 20395473
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