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Addgene

hOtub1
(Plasmid #26959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProEx
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Otub1
  • Alt name
    Otubain1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    816
  • GenBank ID
    NM_017670
  • Entrez Gene
    OTUB1 (a.k.a. HSPC263, OTB1, OTU1)
  • Tag / Fusion Protein
    • His6 (TEV cleavable) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13/pUC reverse 23bp
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hOtub1 was a gift from Cynthia Wolberger (Addgene plasmid # 26959 ; http://n2t.net/addgene:26959 ; RRID:Addgene_26959)

  • For your References section:

    Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1. Wang T, Yin L, Cooper EM, Lai MY, Dickey S, Pickart CM, Fushman D, Wilkinson KD, Cohen RE, Wolberger C. J Mol Biol. 2009 Mar 6. 386(4):1011-23. 10.1016/j.jmb.2008.12.085 PubMed 19211026
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