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Addgene

pDB88
(Plasmid #26958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PMSG346
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    e. coli galactokinase
  • Alt name
    galK
  • Species
    Escherichia coli
  • Insert Size (bp)
    1200
  • Mutation
    cloned under the MOP promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site sbfI (destroyed during cloning)
  • 3′ cloning site sbfI (destroyed during cloning)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDB88 was a gift from Michael Glickman (Addgene plasmid # 26958 ; http://n2t.net/addgene:26958 ; RRID:Addgene_26958)

  • For your References section:

    An improved counterselectable marker system for mycobacterial recombination using galK and 2-deoxy-galactose. Barkan D, Stallings CL, Glickman MS. Gene. 2010 Sep 17. ():. 10.1016/j.gene.2010.09.005 PubMed 20851171
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