Skip to main content
Addgene

pENTR4-SB1 (w789-3)
(Plasmid #26956)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pENTR4
  • Backbone size (bp) 2333
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    no insert
  • Tag / Fusion Protein
    • SB1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (destroyed during cloning)
  • 3′ cloning site Nco I (not destroyed)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SB1 epitope was characterized in Schaeffer et al. 2010 (NAR Apr;38(6):e91)
as part of the SnAvi tag. The codon usage was slightly modified from the original article. The SB1 hybridoma cell line can be ordered from the DevelopmentalStudies Hybridoma bank
http://dshb.biology.uiowa.edu/synaptobrevin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR4-SB1 (w789-3) was a gift from Eric Campeau (Addgene plasmid # 26956 ; http://n2t.net/addgene:26956 ; RRID:Addgene_26956)
Related items:
Commonly requested with: