pKR2.40
(Plasmid #26952)

• Depositing Lab: Geraldine Seydoux
• Publication: Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Backbone

• Vector backbone: pie-1
• Backbone size w/o insert (bp): 12296
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB survival strain
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For expressing GFP-ORF fusions in the germline. This vector does not contain unc-119 sequences and therefore must be co-injected with a marker (typically we use pRF4 in complex array injections - Kelly et al., 1997)