pJH4.66
(Plasmid
#26951)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepie-1
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebeta-tubulin
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SpeciesC. elegans (nematode)
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Entrez Genetbb-1
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
1st generation pie-1 vector
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH4.66 was a gift from Geraldine Seydoux (Addgene plasmid # 26951 ; http://n2t.net/addgene:26951 ; RRID:Addgene_26951) -
For your References section:
Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos. Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G. Dev Biol. 2008 Nov 1. 323(1):76-87. 10.1016/j.ydbio.2008.07.008 PubMed 18692039
