pJH4.52
(Plasmid #26946)

• Depositing Lab: Geraldine Seydoux
• Publication: Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Backbone

• Vector backbone: pie-1
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: histone H2B
• Alt name: F54E12.4
• Species: C. elegans (nematode)
• Entrez Gene: his-58 (a.k.a. CELE_F54E12.4)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

1st generation pie-1 vector

Note that there are some discrepancies between the Addgene QC sequence and the depositor's sequence. They should not affect function.