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Depositing Lab
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Publication
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 26931 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
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Vector typeE.coli bacterial strain
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)EcNR2
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Growth instructionsGrow at 30oC
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MG1655, bla, bio-, lambda-Red1, mutS–, cmR
From the paper: mutS in EcNR1 was replaced with the chloramphenicol resistance gene (cmR cassette).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EcNR2 Strain was a gift from George Church (Addgene plasmid # 26931) -
For your References section:
Programming cells by multiplex genome engineering and accelerated evolution. Wang HH, Isaacs FJ, Carr PA, Sun ZZ, Xu G, Forest CR, Church GM. Nature. 2009 Aug 13. 460(7257):894-8. 10.1038/nature08187 PubMed 19633652