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Depositing Lab
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Publication
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 26930 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Vector typeE.coli bacterial strain
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)EcNR1
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Growth instructionsGrow at 30oC
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MG1655, bla, bio-, lambda-Red1
From the paper: Lambda prophage (from strain DY330; Yu et al. (2000), PNAS 97: 5978-5983) was modified to include the bla gene and introduced into wild-type MG1655 E.coli by P1 transduction at the bioA/bioB gene locus and selected on ampicillin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EcNR1 Strain was a gift from George Church (Addgene plasmid # 26930) -
For your References section:
Programming cells by multiplex genome engineering and accelerated evolution. Wang HH, Isaacs FJ, Carr PA, Sun ZZ, Xu G, Forest CR, Church GM. Nature. 2009 Aug 13. 460(7257):894-8. 10.1038/nature08187 PubMed 19633652