AAV-CAG-ChR2-GFP
(Plasmid #26929)

• Purpose: AAV-mediated expression of ChR2-GFP for neuronal excitation
• Depositing Lab: Edward Boyden
• Publication: Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

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Backbone

• Vector backbone: AAV with CAG promoter
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 5454
• Vector type: Mammalian Expression, AAV ; adeno-associated virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Use recombinase-free E. coli, grow at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ChR2
• Alt name: Channelrhodopsin-2
• Species: C. reinhardtii
• Insert Size (bp): 930
• Promoter: CAG
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry