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Addgene

pBABEpuro KR ATG3
(Plasmid #26927)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy-related 3 (yeast)
  • Alt name
    Atg3
  • Alt name
    APG3
  • Alt name
    Atg3l
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1100
  • Mutation
    Changed Lysine 243 to Arginine.
  • GenBank ID
    NM_026402
  • Entrez Gene
    Atg3 (a.k.a. 2610016C12Rik, APG3, Apg3l, Atg3l, PC3-96)
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Originally cloned from pCMV-SPORT6 vector from Open Biosystems, MMM1013-65573.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEpuro KR ATG3 was a gift from Jayanta Debnath (Addgene plasmid # 26927 ; http://n2t.net/addgene:26927 ; RRID:Addgene_26927)

  • For your References section:

    ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death. Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath J. Cell. 2010 Aug 20. 142(4):590-600. 10.1016/j.cell.2010.07.018 PubMed 20723759
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