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Addgene

pJV62
(Plasmid #26910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLAM12
  • Backbone size w/o insert (bp) 6758
  • Vector type
    Bacterial Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Che9c gene 61
  • Species
    Mycobacteriophage
  • Insert Size (bp)
    1061

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJV62 was a gift from Graham Hatfull (Addgene plasmid # 26910 ; http://n2t.net/addgene:26910 ; RRID:Addgene_26910)

  • For your References section:

    Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets. van Kessel JC, Hatfull GF. Mol Microbiol. 2008 Mar . 67(5):1094-107. 10.1111/j.1365-2958.2008.06109.x PubMed 18221264
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