pJV63
(Plasmid
#26906)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLAM12
- Backbone size w/o insert (bp) 6758
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Vector typeBacterial Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChe9c genes 60-61
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SpeciesMycobacteriophage
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Insert Size (bp)1998
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer sequence
- 3′ sequencing primer sequence (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJV63 was a gift from Graham Hatfull (Addgene plasmid # 26906 ; http://n2t.net/addgene:26906 ; RRID:Addgene_26906) -
For your References section:
Recombineering in Mycobacterium tuberculosis. van Kessel JC, Hatfull GF. Nat Methods. 2007 Feb . 4(2):147-52. 10.1038/nmeth996 PubMed 17179933