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Addgene

pJV53
(Plasmid #26904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLAM12
  • Backbone manufacturer
    Hatfull lab, Addgene plasmid 26908
  • Backbone size w/o insert (bp) 6758
  • Vector type
    Bacterial Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Che9c genes 60-61
  • Species
    Mycobacteriophage
  • Insert Size (bp)
    1998

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJV53 was a gift from Graham Hatfull (Addgene plasmid # 26904 ; http://n2t.net/addgene:26904 ; RRID:Addgene_26904)

  • For your References section:

    Recombineering in Mycobacterium tuberculosis. van Kessel JC, Hatfull GF. Nat Methods. 2007 Feb . 4(2):147-52. 10.1038/nmeth996 PubMed 17179933
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